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Culture HEK293T with poly-lysine

HEK293T poly-lysine

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#1 Benchworker

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Posted 12 April 2012 - 12:34 PM

Hi, All

I am wondering which molecular weight of poly-lysine is more suitable for culturing HEK293T cells on the coverslips, the 70000-150000 or the 150000-300000? The website of Sigma Aldrich just says that MW>70000 is suitable for cell culture.

Thanks for help!

#2 Curtis

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Posted 12 April 2012 - 08:31 PM

I asked the same question 3 years ago in here. We tried different molecular weights and they all worked. They have two main types, L-Lysine and D-lysine. Both must be fine. I used D-lysine.
there are precoated coverslips also available. Sigma has some?! becareful with contamination during coating. use UV.

#3 Benchworker

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Posted 16 April 2012 - 06:58 PM

I asked the same question 3 years ago in here. We tried different molecular weights and they all worked. They have two main types, L-Lysine and D-lysine. Both must be fine. I used D-lysine.
there are precoated coverslips also available. Sigma has some?! becareful with contamination during coating. use UV.

Thanks a lot!




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