2 replies to this topic

[Benchworker]

Hi, All

I am wondering which molecular weight of poly-lysine is more suitable for culturing HEK293T cells on the coverslips, the 70000-150000 or the 150000-300000? The website of Sigma Aldrich just says that MW>70000 is suitable for cell culture.

Thanks for help!

[Curtis]

I asked the same question 3 years ago in here. We tried different molecular weights and they all worked. They have two main types, L-Lysine and D-lysine. Both must be fine. I used D-lysine.
there are precoated coverslips also available. Sigma has some?! becareful with contamination during coating. use UV.

[Benchworker]

Curtis, on 12 April 2012 - 08:31 PM, said:

I asked the same question 3 years ago in here. We tried different molecular weights and they all worked. They have two main types, L-Lysine and D-lysine. Both must be fine. I used D-lysine.
there are precoated coverslips also available. Sigma has some?! becareful with contamination during coating. use UV.

Thanks a lot!