Posted 11 April 2012 - 11:48 AM
First of all, I'm new to molecular biology, so many apologies if I'm full of questions. Second, this is something of a stupid question, so apologies again!
I just finished Maxiprepping, and I accidentally eluted in TE instead of EB. I need to send these samples for sequencing, but I know the EDTA in TE prevents the sequencing reactions from working correctly. How do I go about repurifying the DNA in water or EB so that I may send it out for sequencing?
We do have a PCR purification kit (the Wizard SV kit from Promega), can I use that?
Posted 11 April 2012 - 12:36 PM
Incidentally, I would have done the sequencing on a much smaller prep (e.g. mini) rather than scaling all the way up to a much more expensive maxiprep, before checking.
Posted 11 April 2012 - 02:48 PM