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Problem with protein expression in mammalian cells.


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#1 aneesh vijayan

aneesh vijayan

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Posted 11 April 2012 - 02:51 AM

Hi,

I am trying to express a chimeric fusion protein in mammalian cells (293T, DF-1, BSC-40) using pCDNA 3.1 using Lipofectamine 2000. However there seems to be no expression of my protein. I used the same sequence to purify protein from E.coli and generate recombinant virus (infecting mammalian cells), and both worked with high level of expression. Could anyone suggest a good solution? Thank you.Posted Image




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