Hi,
I have a protein which has both a GST fusion and a 6xHis tag. I was wodnering if I could just purify using either one of those tags (the his OR the GST) and still get good purified protein yield
thanks for your help
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1 reply to this topic
#2
mdfenko
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an elder
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Excellent
Excellent
Posted 11 April 2012 - 07:52 AM
it should be fine as long as the tag for which you select is exposed and accessible.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
Also tagged with one or more of these keywords: purification, protein
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