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Double tagging

purification protein

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#1 LexyD

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Posted 10 April 2012 - 05:21 PM

Hi,

I have a protein which has both a GST fusion and a 6xHis tag. I was wodnering if I could just purify using either one of those tags (the his OR the GST) and still get good purified protein yield

thanks for your help

#2 mdfenko

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Posted 11 April 2012 - 07:52 AM

it should be fine as long as the tag for which you select is exposed and accessible.
talent does what it can
genius does what it must
i do what i get paid to do





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