Hi,
I have a protein which has both a GST fusion and a 6xHis tag. I was wodnering if I could just purify using either one of those tags (the his OR the GST) and still get good purified protein yield
thanks for your help
0
1 reply to this topic
#2
mdfenko
-
- Active Members
-
- 2,288 posts
an elder
83
Excellent
Excellent
Posted 11 April 2012 - 07:52 AM
it should be fine as long as the tag for which you select is exposed and accessible.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
Also tagged with one or more of these keywords: purification, protein
 |
Protocols and Techniques Forums →
SDS-PAGE and Western Blotting →
5x SDS lysis bufferStarted by Guest_JoeSus_* , 31 May 2013  lysis, buffer, protein
|
|
|
|
|
|
Protocols and Techniques Forums →
SDS-PAGE and Western Blotting →
SDS Page Problem: Proteins stuck at stacking lineStarted by Guest_Randy Smith_* , 30 May 2013 sds page, western blot, protein and 2 more...
|
|
|
|
|
|
Protocols and Techniques Forums →
Molecular Biology →
No DNA after PCR product purificationStarted by Guest_tamarine_* , 26 May 2013 PCR product, purification
|
|
|
|
Protocols and Techniques Forums →
Biochemistry →
protein fractionationStarted by Guest_salmonela_* , 09 May 2013 protein, fractionation, bacteria and 2 more...
|
|
|
||
Protocols and Techniques Forums →
General Lab Techniques →
break disulfide bonds to study fluorescenceStarted by Guest_Mulligan_* , 27 Apr 2013 Biophysics Fluorescence and 4 more...
|
|
|
