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cell population doubling

cell culture

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#1 ehernandez4



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Posted 10 April 2012 - 06:17 AM

Hi guys so I am trying to do a population doubling on one of my fibroblast cell lines, and i have been using this formula t= H*ln2/ln(c2/c1), and i tried the website calculator http://www.doubling-time.com, but I noticed I keep getting negative numbers, and that just does not seem right So I am wondering what other formula I could be using instead, and if someone could help answer the reason I may be obtaining negative numbers.

#2 bob1


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Posted 10 April 2012 - 02:21 PM

Have you plotted the growth on a graph? Perhaps your cells don't fit the formulae you are using.

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