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How long to stimulate monocytes by LPS?

CD markers change..

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#1 Paja



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Posted 09 April 2012 - 12:14 PM

Hi everybody,
I am wondering how much time does it take to stimulate peripheral blood monocytes by LPS to see switch in CD markers (from CD14++ to CD14+/CD16+ or CD16++) and other changes in expression of CD80, CD86, CD69 etc. I have already learned I have to stimulate cells by LPS at least 12 better 24 hours to detect cytokine production in supernatants, but how quickly do CD markers react?
Thank you for your kind advices.

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