Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

How long to stimulate monocytes by LPS?

CD markers change..

  • Please log in to reply
No replies to this topic

#1 Paja

Paja

    member

  • Active Members
  • Pip
  • 27 posts
0
Neutral

Posted 09 April 2012 - 12:14 PM

Hi everybody,
I am wondering how much time does it take to stimulate peripheral blood monocytes by LPS to see switch in CD markers (from CD14++ to CD14+/CD16+ or CD16++) and other changes in expression of CD80, CD86, CD69 etc. I have already learned I have to stimulate cells by LPS at least 12 better 24 hours to detect cytokine production in supernatants, but how quickly do CD markers react?
Thank you for your kind advices.
Paja




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.