Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Explain the basic principles of how a cDNA microarray operates?


  • Please log in to reply
2 replies to this topic

#1 MicroNerd

MicroNerd

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 08 April 2012 - 01:17 PM

Explain the basic principles of how a cDNA microarray operates, showing how we can take advantage of some of the fundamental characteristics of the DNA molecule to determine gene expression in tissue samples taken from normal and ‘diseased’ cells?

Can anyone help please?

Edited by MicroNerd, 08 April 2012 - 01:33 PM.


#2 RNA68

RNA68

    member

  • Members
  • Pip
  • 1 posts
2
Neutral

Posted 08 April 2012 - 01:30 PM

A DNA array typically a glass microscope slide that has been specially treated to remove all moisture then a specific silane monolayer is applied e.g. Amino Propyl Tri Ethoxy Silane. The DNA dots, really oligonucleotides, half of a DNA spiral are applied to the silane surface with a piece of equipment. If the silane has been applied correctly the dot sticks for years and the next step of the operation is to apply samples of different oligonucleotides to the treated slide. The applied dots are a range of DNA types and the other half of the DNA helix can only cling to the correct oligo. If the smple oligo,s have a fluorescent dye applied then only where they react will there be any fluorescence. By shining a light at the slide and seeing a fluorescence at say the third row and the fourth dot in you can state that that dot represents the sample DNA. Knowing the sequence of dots allows you to know instantly the disease that is represented in the sample.



#3 MicroNerd

MicroNerd

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 08 April 2012 - 01:34 PM

Thank you very much for the helpful answer.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.