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how to prepare TBE 10x

how to prepare TBE

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4 replies to this topic

#1 blondy11

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Posted 06 April 2012 - 10:58 AM

I'm wondering how to prepare this buffer (TBE 10x)?

I need to know if final volume of this buffers is 1liter, when i dissolve each component and add 1 liter the volume is bigger than 1liter. When I make buffers how much water i should use? Can I correct the volume of buffer with components to 1liter or i should add exactly 1 liter to the components. In second case the volume of buffer will be bigger than 1 liter. It is silly question but ?

#2 casandra

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Posted 06 April 2012 - 11:14 AM

I'm wondering how to prepare this buffer (TBE 10x)?

I need to know if final volume of this buffers is 1liter, when i dissolve each component and add 1 liter the volume is bigger than 1liter. When I make buffers how much water i should use? Can I correct the volume of buffer with components to 1liter or i should add exactly 1 liter to the components. In second case the volume of buffer will be bigger than 1 liter. It is silly question but ?

You have to dissolve in less than the final volume, in your case, if you're preparing 10x TBE stock solution in 1 liter, dissolve all the components first in 700 to 800 ml, then complete to a liter.
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#3 blondy11

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Posted 07 April 2012 - 08:38 AM

So there is no other way to do it or will be incorrect?

#4 casandra

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Posted 07 April 2012 - 10:29 AM

So there is no other way to do it or will be incorrect?

you're preparing a solution here (a certain amount of solute in a certain volume of solvent) so do you think you will have the same concentration if you dissolve 108 g of Tris base in 1 liter and let's say, in 1.2 liters? If you ended with with a lot more final volume than you initially computed for, then just recompute and readjust for a bigger volume.
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#5 Deepak Anand

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Posted 09 April 2012 - 06:26 AM

There must be a book by name "Molecular Cloning" by Sambrook. There you will get all the molecular biology reagent preparation and standard protocols. Y need to see the Volume 3 Appendices (Page no A1 onwards)
Here I will tell you
5x stock for 1Liter
54gm Tris base
27.5gm boric acid
dissolve them and add
20 ml of 0.5 M EDTA solution
final volume to 1 liter




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