Purifying recombinant protein from pellet?
Posted 04 April 2012 - 03:51 AM
I am producing a protein in E.coli and the problem is that it is not soluble. For purification I am using GE healthcare spin trap for purifying the His tagged protein (http://www.gelifesci...moduleid=166507).
I decided to test if you can purify the protein from the pellet so I just resuspended the pellet to 0,5 ml buffer and just purified the protein using the kit and got nice results on SDS-page.
Can this protein be functional?
Posted 05 April 2012 - 01:21 PM
Posted 09 April 2012 - 03:01 PM