Posted 04 April 2012 - 06:07 AM
I have done purification with RNeasy a few times, and as a result I have seen that I could only obtain 1/2-1/3 of the RNA at the end. So you will get your RNA diluted even more. Buffer RLT is required for lysis of the cells. Since you have no cells in your case, I don't think it is very crucial to have high amount of RLT. However, I never done this part with low amount of RLT, so I am not 100% sure. Maybe you can try this with lower amounts of RLT with some expendable RNAs, if you have enough columns.