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qpcr strange curves

qPCR real time PCR curve curves

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#1 Assaf Assis

Assaf Assis


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Posted 03 April 2012 - 10:38 PM

HI everyone !

2 days ago i was making a qPCR reaction.
i checked 3 genes and i use as gyrA as positive control.

when i got the results, i noticed that 2 from the 4 genes has strange curves.
from some reason, only the dupicates of the gene of interest shows not very logaritmic curve.
i thought it could be the primers, but the standard curve works fine.
maybe the cyber recognize diffrently the CDNA and the DNA ?

i attach here word file with the 4 genes.
the up-right sample is the positive gene and works fine.
the rest are not.

thanks a lot !!!!

i'm sorry, but i cant upload the file, so please send me your email and i will send it right away.

Edited by Assaf Assis, 03 April 2012 - 10:44 PM.

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