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Calculation of transfection efficiency for Dual Luciferase Assay

luciferase assay transfection

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#1 hermit

hermit

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Posted 03 April 2012 - 09:52 PM

Dear all,

Since I've got the data of dual luciferase assay only (using Promega Dual Luciferase Reporter System), I would like to ask how to calculate the transfection efficiency.

Assume here are some of my experimental data:

1st reading (Renilla) 2nd reading (Firefly)
pGL4-74 / pGL4-10 4.20 2.67
pGL4-74/ pGL4-23 0.81 0.73
pGL4-74/ pGL4-13 7.61 120.9
pGL4-74/ ddH2O 0.02 0.02
ddH2O only 0.01 0.01

pGL4-74 is used to normalize the transfection; reading of pGL4-13 is a max expression; pGL4-13 is used for checking the basal expression level.

Thx,
Hermit





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