Hi fellow chippers!
As you probably all already know, there have been some recently published protocols on how to perform ChIP-seq on very few cells (~10,000 cells or less):
Paper 1: http://www.nature.co...nmeth.1478.html
Corresponding Nature Protocols: http://www.nature.co...t.2011.402.html
Paper 2: http://www.nature.co...ml#/ref-link-11
Corresponding Nature Protocols: http://www.nature.co...t.2011.447.html
I had previously attempted the amplification part of the first protocol (WGA method), but was unable to reproduce their results, not even close... I have never tried the T7 method in paper 2.
My question is if anyone has successfully used any of these protocols for their ChIP-seq experiments? If so, I'd love some tips!
0
Small cell number ChIP-seq protocols - has anyone tried them?
Started by angelawu, Apr 03 2012 04:08 PM
ChIP-seq Protocol WGA amplification
No replies to this topic
Also tagged with one or more of these keywords: ChIP-seq, Protocol, WGA, amplification
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