Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

LC3 Autophagy Huh7

LC3 Autophagy Western blot Huh7

  • Please log in to reply
1 reply to this topic

#1 HOYAJM

HOYAJM

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 159 posts
12
Good

Posted 03 April 2012 - 12:07 PM

I am looking at autophagy levels using polyclonal LC3 antibody in Huh7 cells. My expectation is that little/no autophagy is occuring in Huh7 cells. However, when I performed a western blot I had very dense LC3I and LC3II bands indicitive of autophagy. Why is a Huh7 cell line (that appears healthy in culture) undergoing autophagy? I am going to treat with an autophagy inhibitor to determine if I can decrease the level of LC3II, but does anybody have an explanation as why I see high levels of both LC3I and LC3II?

Thanks

#2 HOYAJM

HOYAJM

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 159 posts
12
Good

Posted 09 April 2012 - 10:13 AM

Anybody? We use 10% serum, seems to be the standard, autophagy can be induced when serum is low





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.