Hello,
i would like to know if it is possible to measure a cell cycle stained with DAPI with a violet (405nm) laser instead of a UV lamp? I know that concerning the excitation maximum of DAPI, UV would be much better than a violet laser, but since DAPI is very bright, are there people using the violet laser when a UV lamp is not available?
Thanks in advance.
0
violet laser (405nm) and cell cycle staining with DAPI
Started by Slu, Apr 03 2012 11:26 AM
violet 405 cell cycle DAPI
1 reply to this topic
#2
Curtis
-
- Global Moderators
-
- 981 posts
Metaller Scientist
45
Excellent
Excellent
Posted 03 April 2012 - 06:59 PM
I found DAPI very detectable under other filters too. I just had problem with UV because after a while under exposure the DAPI-stained nuclei turned green. It was like they were burning 
Edited by Curtis, 03 April 2012 - 07:00 PM.
Also tagged with one or more of these keywords: violet, 405, cell cycle, DAPI
Student Forums (for study, homework and non technical questions) →
-Cell Biology- →
Immunocytohistochemistry & DAPIStarted by Guest_awesomeosaurus_* , 25 Feb 2013  DNA, DAPI, STUFF, AHHHHH, LPS
|
|
|
||
Protocols and Techniques Forums →
Flow Cytometry →
Heeeeeelp..cell cycle sample..is it possible to store it?Started by Guest_shs_* , 19 Jan 2013 FACS, cell cycle
|
|
|
||
Protocols and Techniques Forums →
Flow Cytometry →
DAPI and Alexa Fluor 488Started by Guest_jeee_* , 06 Jun 2012 DAPI, 488, green, Hoechst and 1 more...
|
|
|
||
Protocols and Techniques Forums →
Cell Biology →
Nuclei staining background in collagen matrixStarted by Guest_rz806_* , 17 Feb 2012 Nuclei staining, collagen matrix and 3 more...
|
|
|
