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violet laser (405nm) and cell cycle staining with DAPI

violet 405 cell cycle DAPI

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#1 Slu

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Posted 03 April 2012 - 11:26 AM

Hello,
i would like to know if it is possible to measure a cell cycle stained with DAPI with a violet (405nm) laser instead of a UV lamp? I know that concerning the excitation maximum of DAPI, UV would be much better than a violet laser, but since DAPI is very bright, are there people using the violet laser when a UV lamp is not available?
Thanks in advance.

#2 Curtis

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Posted 03 April 2012 - 06:59 PM

I found DAPI very detectable under other filters too. I just had problem with UV because after a while under exposure the DAPI-stained nuclei turned green. It was like they were burning Posted Image

Edited by Curtis, 03 April 2012 - 07:00 PM.






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