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Looking for a method to downregulate estrogen receptor alpha mRNA in MCF-7 cells

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#1 spanishninja



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Posted 03 April 2012 - 10:47 AM

Hello. I am trying to develop an assay to measure the downregulation of ER-alpha in a cell line that expresses it (preferably MCF-7). For the people who are familiar with this gene, how is it usually regulated at the RNA level? I've read certain papers that state that ER-alpha mRNA is regulated post-transcriptionally through affecting RNA stability. Is this true? What type of treatment would be best to downregulate its mRNA levels? Thanks very much.

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