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Competent cells question: JM101 vs DH5alpha

DH5alpha JM101 Transformation

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#1 Steve Hwang

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Posted 03 April 2012 - 09:46 AM

I've been having difficulty tracking down E. coli JM101 in my lab's freezer inventory. I'm trying to replicate a published experiment that utilizes JM101 as a host for intermediate plasmids (pBluescript + cloned genes + resistance marker), and I'm wondering if I can just use DH5alpha as host to replicate these intermediate plasmids. Are there any issues with doing this?

Edited by Steve Hwang, 03 April 2012 - 10:02 AM.


#2 phage434

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Posted 03 April 2012 - 03:54 PM

I'm guessing it would be easier with DH5a. JM101 has an intact E. coli restriction system, which will reduce transformation efficiency for some constructs. It also is recA+, which may make some constructs less stable. Is this a paper from 1980?

#3 Steve Hwang

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Posted 04 April 2012 - 09:18 AM

It's a paper from 2003. But thanks for the info!





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