2 replies to this topic

[Steve Hwang]

I've been having difficulty tracking down E. coli JM101 in my lab's freezer inventory. I'm trying to replicate a published experiment that utilizes JM101 as a host for intermediate plasmids (pBluescript + cloned genes + resistance marker), and I'm wondering if I can just use DH5alpha as host to replicate these intermediate plasmids. Are there any issues with doing this?

Edited by Steve Hwang, 03 April 2012 - 10:02 AM.

[phage434]

I'm guessing it would be easier with DH5a.  JM101 has an intact E. coli restriction system, which will reduce transformation efficiency for some constructs.  It also is recA+, which may make some constructs less stable.  Is this a paper from 1980?

[Steve Hwang]

It's a paper from 2003. But thanks for the info!