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serum microRNA qPCR normalization

serum microRNA qPCR

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#1 wgdequincey



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Posted 02 April 2012 - 03:42 AM

Hello there,

Anyone out there who are working on serum microRNAs?

I run a qPCR screening and my normalization control seemed to be degraded in the serum.

Does anyone knows how to use total rna for normalization? Right now that's all I can think of doing but in the future I'll spike my samples with standard microRNA.


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