Could somebody please explain me how to distinguish between homozygous and heterozygous by PCR and/or Southern blot?
I know that when an individual is homozygous for a gene, it will have only one PCR band. I found some textbook saying that a homozygous could also have 2, 4 PCR bands!
How is this possible?
In the figure attached is an example.
It is said:
With one band: homozygous
2 bands: also homozygous
3 bands: heterozygous
How 2 bands make homozygous, if we speak about the same gene?
If there is only a substitution of one base (to make a restriction site), does this change the size of gene?
Edited by Biog, 02 April 2012 - 03:33 AM.