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Proving v-oncogenes are responsible for tumorigenicity

cDNA vs RNA

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#1 Cytophile

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Posted 01 April 2012 - 01:03 PM

So I have to prove that v-oncogenes, in case of RNA tumor viruses, are directly responsible for tumorigenicity.

I plan on
1. using an expression vector, transfect viral genetic sequences into cells and see if they proliferate.
2 I'm next going to use either antisense RNA or anti-protein abs to inhibit growth if there is any caused by the RNA tumor virus.

My question is , when I transfect viral genes should I use RNA or cDNA?

Thanks.

#2 bob1

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Posted 03 April 2012 - 12:38 PM

You would use it in a form that will result in the transfection having the same effect as the virus entering the cell...




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