So I have to prove that v-oncogenes, in case of RNA tumor viruses, are directly responsible for tumorigenicity.
I plan on
1. using an expression vector, transfect viral genetic sequences into cells and see if they proliferate.
2 I'm next going to use either antisense RNA or anti-protein abs to inhibit growth if there is any caused by the RNA tumor virus.
My question is , when I transfect viral genes should I use RNA or cDNA?
Proving v-oncogenes are responsible for tumorigenicitycDNA vs RNA
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