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Requesting basic protocols on cell sorting


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#1 Chelo

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Posted 01 April 2012 - 07:06 AM

Hi everybody,

I have to separate epithelial cells in urine by FACS. I have a good labelled antibody for a cell surface marker but I don´t know anything about cytometry and cell sorting. That is why I would be very grateful if someone could advise me on how I should label the cells with this antibody. I normally use PBS-tween for Northern-blots or ELISAs but in this case, I want to recover the cells intact. So I guess I have to avoid detergents, right?
Thanks a lot in advance!

#2 Curtis

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Posted 01 April 2012 - 06:59 PM

Every FACS machine has a protocol for that. Your machine must have a manual. Have you studied yours?

Edited by Curtis, 01 April 2012 - 06:59 PM.





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