I have two questions for my final exam and really don't know where to start hope that you may be able to help
35]1. The protein product of the gene xyz1 is produced in a hepatic cell line. Outline, with a justification, the procedure you would use to clone the coding DNA sequence of the xyz1 into a DNA cloning vector for further studies. For each step of the cloning procedure review the principles behind the methodology described.
35]2. The coding DNA sequence for Protein Y has been cloned into the pUC8 vector. Explain why this vector is unsuitable to produce a recombinant protein and describe 2 alternative vectors that would allow you to express the protein in prokaryotic and mammalian cells (ie discuss one example each of a prokaryotic and a mammalian expression vector). Review the benefits and disadvantages of both expression systems
35]I'm guessing they might both have something to do with introns but really need some help