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#1 Vsmith1



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Posted 30 March 2012 - 09:24 AM

Hey there,

I have two questions for my final exam and really don't know where to start hope that you may be able to help Posted Image

35]1. The protein product of the gene xyz1 is produced in a hepatic cell line. Outline, with a justification, the procedure you would use to clone the coding DNA sequence of the xyz1 into a DNA cloning vector for further studies. For each step of the cloning procedure review the principles behind the methodology described.

35]2. The coding DNA sequence for Protein Y has been cloned into the pUC8 vector. Explain why this vector is unsuitable to produce a recombinant protein and describe 2 alternative vectors that would allow you to express the protein in prokaryotic and mammalian cells (ie discuss one example each of a prokaryotic and a mammalian expression vector). Review the benefits and disadvantages of both expression systems

35]I'm guessing they might both have something to do with introns but really need some help

35]Thanks Posted Image


#2 AndrewT



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Posted 04 May 2012 - 02:30 AM

Hi Veronica, you might find your lecture notes a good place to start on these questions, after all anyone could be posting replys here! AndrewT

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