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Plasmid vs cDNA amplification by PCR

plasmid DNA cDNA

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2 replies to this topic

#1 lmh8m



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Posted 29 March 2012 - 06:33 AM

why is it easier to amplify plasmid DNA than it is to amplify cDNA?

#2 mdfenko


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Posted 29 March 2012 - 06:44 AM

standard primers and, therefore, well defined conditions.
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#3 Trof


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Posted 29 March 2012 - 08:58 AM

And when talking about the same sequence in plasmid and in cDNA, plasmid DNA contains only huge number of copies of relatively small, not-complex stable molecule, but cDNA is a mix of all transcriptome of cells, RNA maybe degraded before RT and thus contain incomplete molecules, cDNA may degrade too, plus contaminants from RT blocking PCR.
And I'm sure I left something out.

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