2 replies to this topic

[lmh8m]

why is it easier to amplify plasmid DNA than it is to amplify cDNA?

[mdfenko]

standard primers and, therefore, well defined conditions.

[Trof]

And when talking about the same sequence in plasmid and in cDNA, plasmid DNA contains only huge number of copies of relatively small, not-complex stable molecule, but cDNA is a mix of all transcriptome of cells, RNA maybe degraded before RT and thus contain incomplete molecules, cDNA may degrade too, plus contaminants from RT blocking PCR.
And I'm sure I left something out.