Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

If i accidentally froze my PBS

pbs hela frozen

  • Please log in to reply
1 reply to this topic

#1 Grace Swickley

Grace Swickley


  • Members
  • Pip
  • 1 posts

Posted 29 March 2012 - 06:16 AM

If i accidentally froze my PBS and used this frozen PBS on HELA cells and then wanted to do lysis to my cells. How would this in fact effect my cells? and the proteins inside? Thank you :)

#2 mdfenko


    an elder emeritus

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,266 posts

Posted 29 March 2012 - 06:21 AM

as long as the pbs was fully defrosted and mixed prior to use there should be no difference from using pbs that has not been frozen, unless you have freeze sensitive additives in your solution.
talent does what it can
genius does what it must
i do what i get paid to do

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.