Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Shear-induced damage of plasmid by vortexing

plasmid DNA damage vortexing

  • Please log in to reply
1 reply to this topic

#1 mbgene

mbgene

    member

  • Active Members
  • Pip
  • 5 posts
0
Neutral

Posted 29 March 2012 - 06:12 AM

Hi everyone

I have a general question regarding handling of plasmid DNA.

What's the best/most optimal way to mix plasmid DNA? By vortexing, by inverting, by pipetting up and down, or???

And following this question: does vortexing damage plasmid DNA? If yes, does it concern all sizes of plasmids, or only concern plasmid larger than a certain size?

Would be great to get your answers/experience.

Thanks a lot!

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,674 posts
396
Excellent

Posted 29 March 2012 - 11:30 AM

If you mean isolated plasmid DNA (i.e. not during the extraction process), then any mixing will work fine.

Plasmids should not be damaged by any of the mixing methods you mention, they are too small. I don't know the size cut-off, but in theory at least, bacterial genomic DNA can be sheared by vortexing.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.