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Can anyone help me to identify what line is that???

2D electrophoresis gel

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4 replies to this topic

#1 new learner

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Posted 28 March 2012 - 08:24 PM

Does anyone know what line is at the bottom part of the gel??Is it occur because of protein degradation??? I am confused. Thank you for your help.~^_^~

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#2 mdfenko

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Posted 29 March 2012 - 06:31 AM

the lines you are seeing are not unusual. they are caused by buffer fronts which migrate through the gel during the run.
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genius does what it must
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#3 new learner

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Posted 29 March 2012 - 05:10 PM

but y is the intensity is much higher than b4??but I use the same buffer.... Yesterday I run another gel but the spots are much less than this....do u know y???although they are from different samples but supposely the outcome will give similarities....

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#4 mdfenko

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Posted 30 March 2012 - 08:29 AM

the gels show similarities but it looks like you ran the second gel longer and the buffer fronts ran off (nearly completely).

sometimes the buffer fronts are caused by aging components (often the sds, if you see the bromphenol blue run diffuse and sharpen at the end).
talent does what it can
genius does what it must
i do what i get paid to do

#5 new learner

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Posted 01 April 2012 - 04:42 PM

ok...thank you...today i will run another gel n see the outcome...hopefully it gives positive results....





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