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3h dopamine assay !! Help


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4 replies to this topic

#1 CKtong

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Posted 28 March 2012 - 07:01 PM

hi guys,
I am working on settip up 3h dopamine assay for analysis of the culture primary/ cell line culture experiment. We have a Microbeta trilux in the lab. however it is very limited information available online. Who has a detail protocol to share?

Thanks :)

#2 mdfenko

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Posted 29 March 2012 - 05:33 AM

if you are requesting information on the use of the microbeta trilux then you can get instruction at these sites:

[url="http://www.whitelabs.org/instrument%20manuals/Trilux/MicroBeta%20v4.0%20user%20manual%20bs.pdf"]microbeta v4.0 user manual[/url] (pdf)

[url="http://assay.nih.gov/assay/index.php/Section16:Instrumentation"]assay guidance wiki[/url]

dopamine assay information can be found through google scholar:

[url="http://scholar.google.com/scholar?q=3h+dopamine+assay&hl=en&as_sdt=0&as_vis=1&oi=scholart&sa=X&ei=FGR0T6m1C8e8twezm-CNBg&sqi=2&ved=0CB4QgQMwAA"]search page[/url]
talent does what it can
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#3 CAT

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Posted 29 March 2012 - 06:32 AM

Hey, you need provide me more details. R u doing [3H]DA binding to receptors?

#4 CKtong

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Posted 29 March 2012 - 06:48 AM

Hi CAT
I like to set up (3H]DA uptake assay to measure dopamine transporter uptake in neuronal cell line and primary mesencephalic culture.

#5 CAT

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Posted 29 March 2012 - 09:26 AM

http://www.sciencedi...014299904011987




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