3h dopamine assay !! Help
Posted 28 March 2012 - 07:01 PM
I am working on settip up 3h dopamine assay for analysis of the culture primary/ cell line culture experiment. We have a Microbeta trilux in the lab. however it is very limited information available online. Who has a detail protocol to share?
Posted 29 March 2012 - 05:33 AM
[url="http://www.whitelabs.org/instrument%20manuals/Trilux/MicroBeta%20v4.0%20user%20manual%20bs.pdf"]microbeta v4.0 user manual[/url] (pdf)
[url="http://assay.nih.gov/assay/index.php/Section16:Instrumentation"]assay guidance wiki[/url]
dopamine assay information can be found through google scholar:
genius does what it must
i do what i get paid to do
Posted 29 March 2012 - 06:32 AM
Posted 29 March 2012 - 06:48 AM
I like to set up (3H]DA uptake assay to measure dopamine transporter uptake in neuronal cell line and primary mesencephalic culture.