4 replies to this topic

[CKtong]

hi guys,
I am working on settip up 3h dopamine assay for analysis of the culture primary/ cell line culture experiment.  We have a Microbeta trilux in the lab. however it is very limited information available online. Who has  a detail protocol to share?

Thanks

[mdfenko]

if you are requesting information on the use of the microbeta trilux then you can get instruction at these sites:

microbeta v4.0 user manual (pdf)

assay guidance wiki

dopamine assay information can be found through google scholar:

search page

[CAT]

Hey, you need provide me more details. R u doing [3H]DA binding to receptors?

[CKtong]

Hi CAT
I like to set up (3H]DA uptake assay to measure dopamine transporter uptake in neuronal cell line and primary mesencephalic culture.

[CAT]

http://www.sciencedi...014299904011987