Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

3h dopamine assay !! Help


  • Please log in to reply
4 replies to this topic

#1 CKtong

CKtong

    member

  • Active Members
  • Pip
  • 17 posts
0
Neutral

Posted 28 March 2012 - 07:01 PM

hi guys,
I am working on settip up 3h dopamine assay for analysis of the culture primary/ cell line culture experiment. We have a Microbeta trilux in the lab. however it is very limited information available online. Who has a detail protocol to share?

Thanks :)

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,704 posts
123
Excellent

Posted 29 March 2012 - 05:33 AM

if you are requesting information on the use of the microbeta trilux then you can get instruction at these sites:

microbeta v4.0 user manual (pdf)

assay guidance wiki

dopamine assay information can be found through google scholar:

search page
talent does what it can
genius does what it must
i do what i get paid to do

#3 CAT

CAT

    member

  • Active Members
  • Pip
  • 24 posts
0
Neutral

Posted 29 March 2012 - 06:32 AM

Hey, you need provide me more details. R u doing [3H]DA binding to receptors?

#4 CKtong

CKtong

    member

  • Active Members
  • Pip
  • 17 posts
0
Neutral

Posted 29 March 2012 - 06:48 AM

Hi CAT
I like to set up (3H]DA uptake assay to measure dopamine transporter uptake in neuronal cell line and primary mesencephalic culture.

#5 CAT

CAT

    member

  • Active Members
  • Pip
  • 24 posts
0
Neutral

Posted 29 March 2012 - 09:26 AM

http://www.sciencedi...014299904011987




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.