Cloning in pETDuet-1
Posted 28 March 2012 - 01:39 PM
I was wondering if anyone here has tried cloning in the pDuet-1(5420 bp) vector. I will be cloning two target genes and was wondering what is the capacity of the vector i.e. what is the max size of the target genes that I can put in there?
Posted 29 March 2012 - 10:45 PM
I have done cloning in pDuet-1 vector, but it was long back. I remember the sizes of the genes were not that big, one was around 500bp and the other was around 1.2 kb. Maximum size of the gene that u can put.....I have no idea...
Posted 30 March 2012 - 06:34 AM