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Columns for ssDNA oligo purification

DNA Purification isotope end labelling

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#1 stelane

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Posted 28 March 2012 - 12:20 PM

Just wondering what brand of spin columns other labs are using for purification of ssDNA after end labelling?

We currently use GE Healthcare's Illustra Microspin G-25 columns for recovery of a 39-mer probe after labelling with isotope, but were wondering if there are any cheaper/better options out there?

Thanks.

#2 Trof

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Posted 28 March 2012 - 02:22 PM

We used Quick Spin colums from Roche, but I would doubt that they're cheaper.
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#3 phage434

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Posted 28 March 2012 - 05:44 PM

You can make your own with Sephadex G-25 and an empty filter column.  Biorad makes empty columns.





Also tagged with one or more of these keywords: DNA, Purification, isotope, end labelling

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