I 'm planning to pull-down the interacting proteins of my 6XHis-tagged protein with anti-His beads. But I can find a few agarose beads for His antibodies. What's the difference between magnatic and agarose beads?
Thanks!
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What's the difference between megnatic beads and agarose beads to IP His-tag
Started by Michelle1, Mar 28 2012 10:58 AM
His beads
3 replies to this topic
#2
bob1
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Posted 28 March 2012 - 07:49 PM
The magnetic beads use a magnet to pull them out of suspension, which is gentler than centrifugation, they can also be centrifuged though. The agarose (sepharose) beads can only be pulled down by centrifugation.
#3
Michelle1
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Posted 29 March 2012 - 08:42 AM
Thanks!
If I'm about to do IP to look for interacting proteins. Is magnetic bead a good choice? I see very few agarose beads for His-tag but a lot magnetic and nickel NTA something which I never worked with before.
If I'm about to do IP to look for interacting proteins. Is magnetic bead a good choice? I see very few agarose beads for His-tag but a lot magnetic and nickel NTA something which I never worked with before.
#4
bob1
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Posted 29 March 2012 - 12:07 PM
It will work fine. NiNTA is usually used for column based preparations.
