Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

What's the difference between megnatic beads and agarose beads to IP His-tag

His beads

  • Please log in to reply
3 replies to this topic

#1 Michelle1

Michelle1

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 28 March 2012 - 10:58 AM

I 'm planning to pull-down the interacting proteins of my 6XHis-tagged protein with anti-His beads. But I can find a few agarose beads for His antibodies. What's the difference between magnatic and agarose beads?

Thanks!

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,674 posts
396
Excellent

Posted 28 March 2012 - 07:49 PM

The magnetic beads use a magnet to pull them out of suspension, which is gentler than centrifugation, they can also be centrifuged though. The agarose (sepharose) beads can only be pulled down by centrifugation.

#3 Michelle1

Michelle1

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 29 March 2012 - 08:42 AM

Thanks!

If I'm about to do IP to look for interacting proteins. Is magnetic bead a good choice? I see very few agarose beads for His-tag but a lot magnetic and nickel NTA something which I never worked with before.

#4 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,674 posts
396
Excellent

Posted 29 March 2012 - 12:07 PM

It will work fine. NiNTA is usually used for column based preparations.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.