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Need help w question about cloning in pUAST

heat shock cloning cloning pUAST heat shock pCASPER

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#1 Juanita_G

Juanita_G

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Posted 28 March 2012 - 10:16 AM

Hello,

I need to get a Plasmid with an insert that could be heat-shock induced expressed(and only then).
My insert has the restriction sites for NotI and XbaI, so I found the pUAST or the hs-pCasper Vector for my cloning.


Am I right with my findings of these 2 Vectors? Is one better than the other?
Has anybody experienced difficulties at cloning with these?


Thanks for your help!
Juanita





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