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Does DNase work at 8 degree?


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#1 BMF



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Posted 28 March 2012 - 08:33 AM

I am working in Genome center of Mcgill University and there are two post doc persons who have obtained their PhD degrees from France and UK respectively. This one from UK has learned to do RNA extract in two months ago, I mean he has just learned to do RNA extract from two months ago.

When I said that DNases work at 8 degree, they laughed at me. They usually make fun of me specially when I am not in the Lab.
I want to add nuclear extract to my beads which have bounded oligo and shake it for 2hour at 4 degree.
What is your idea?
I am new PhD student.

Edited by memari, 28 March 2012 - 08:41 AM.

#2 bob1


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Posted 28 March 2012 - 12:17 PM

DNases will work at 8 degrees C, but the action will be fairly slow/almost non-existent in the two hour time-frame that you are proposing.

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