Posted 31 March 2012 - 07:14 AM
I'd revert to the original formula for M9 medium. Your approach seems to be a compromise between CAP as sole source of carbon and energy and CAP metabolism in context of a growing culture. If you want the former, you should increase the CAP concentration - 100 ppm is hardly substrate level. Assuming you chose the low level to avoid likely inhibition, it would serve you better to start with a more complete medium with increased CAP then modifying conditions progressively - conceptually shooting for tolerance -> resistance -> metabolism -?> substrate. Diminshing nutrients while increasing CAP.
And why work with such large volumes? Filter down to something more workable.