I am using XL10 Gold cells, I grow out the transformation mixture for 1h at 37C in 500 uL NYZ broth. Then I plate 150 uL of the mixture on LB+Amp plates
I will be inoculating LB+Amp with the colonies and will see if they grow with antibiotic. But in the meantime I was just curious if people have had the same experience and found that single colonies on a plate after SDM could in fact contain the mutation.
Edited by HOYAJM, 27 March 2012 - 07:00 AM.