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Plant Thylakoid Quantification. How?

Thylakoids

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#1 Popeye

Popeye

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Posted 26 March 2012 - 07:26 AM

Ok, so I am not absolutely sure that this os the place to post my request for help but, I really am desperate.

I am finishing my Master's Degree in Portugal.

I have to quantify the amount of thylakoids I have extracted from a few green plants.

I already know that Chlorophyll quantification is the best method but...
which of the several Chlorophyll quantification methods would you advise??
And more importantly:
- What is the correlation between chlorophyll quantity and thylakoid quantity??
I mean, imagine I get a concentration of 1mg of chlorophyll per liter of extract....what quantity of thylakoid membranes does that correspond to??

I really need help here.
Thank you anyone!




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