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I would like to ask, whether you have experiences with isolating both DNA and RNA from the same tissue. I am working with plants. I tried some commercial kits - basicly, the RNA isolation kit is used with extra buffers for extracting DNA. The RNA extraction part works perfectly, but I have very low amounts of DNA. Thus, it makes no sense to use this approach for isolation of both nucleic acids. I was then thinking about isolating DNA and RNA separately, with the specific kit or non-kit method. The problem is, how to split my tissue, since I would like to have nucleic acids from the same tissue. My idea was to:
1.) homogenize the sample - I am always using leaves frozen in nitrogen, and homogenizing with metal beads in a homogenizer.
2.) add a lysis buffer to this frozen powder, make the sample liquid, and then split into two parts, and continue with the extracting DNA and RNA separately.
However, the question is, whether I can use the lysis buffer from e.g. RNA kit, and then to continue with isolating DNA by DNA extraction kit, or eventually vice versa, i.e. using lysis buffer from DNA extr. kit, and continue with isolating RNA by RNA extr. kit. I contacted the manufacturer of the kit I was using (Macherey Nagel), and they said that this is not possible, since the lysis buffers for DNA and RNA extraction are different. I tried anyway, and it somehow works, but I would like to have a kind of representative results
I am wondering whether there is some better way how to split my samples into two parts, so I can then proceed to DNA and RNA extraction without affecting it negatively? Thanks a lot in advance
