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how to do RT-PCR for16srRNA of a bacteria?

Started by joy123, Mar 25 2012 07:24 PM

RT-PCR 16srRNA

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#1 joy123

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Posted 25 March 2012 - 07:24 PM

Hi, I want to compare the 16sRNA level of a certain bacteria between patients and healthy by Real Time-PCR. I am a new starter and hope to get some advice.

1. How can I find the 16srRNA to design pathogen specific primers?

2.what general procedures should I go through?

3. My interested bacteria is a G+. How should I extract the bacteria DNA? The sample contains mammal cells, G+ and G- bacteria.

Thanks a lot! Posted Image

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