Extra genotype in floxed mouse breeding
Posted 24 March 2012 - 02:43 PM
My supervisor had explained how this extra band in generated in <5% of mice, and it was due to some kind of recombination event rather than a fault with the PCR. I've since forgotten how this extra band occurs and she's not answering her email (to me) anymore :s
Anybody have any idea what I'm talking about? It's happening in my new lab and I want to sound smart
Posted 26 March 2012 - 02:05 PM
Posted 26 March 2012 - 06:07 PM
*but I'm not sure, it was years ago when it was mentioned to me so I'm pretty hazy.
We just cull the weird ones, so I don't really need to know what it is - it's just nice to know, you know?
Edited by batteryhorsestaple, 26 March 2012 - 06:08 PM.
Posted 11 December 2012 - 01:20 PM
I am currently genotyping some transgenic mice for the presence of a floxed allele and i have observed something almost identical indeed i have just today posted a question similar to yours on this forum. In my case i have now used three different primer sets which flank the floxed insertion allele and i often get a third allele which is 300bp longer than wild type but about 1000bp shorter than the floxed allele. I had thought something weird must have happened during recombination but i cant get a satisfactory answer so hopefully somebody may be albe to find an answer as it would be very much appreciated. I am intending to cut out a third allele band and get it sequenced just to see what exactly it is as i hate not knowing.