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How to lysis cells for an RNA extraction

RNA cell lysing methods

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#1 ehernandez4

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Posted 23 March 2012 - 12:16 PM

HI guys

So I have a couple of cell lines in culture, and i am trying to collect the cells for an RNA extraction, but not quite sure how to do a cell lysis for this particular thing. do you guys have any tips or protocols that i can try out to see, and begin doing that?

#2 memari

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Posted 23 March 2012 - 07:49 PM

just add trizol, then collect it in a vial then add chloroform to it. then isopropanol then 70 % alcohol then dry it and add water and use it.
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Babak Memari





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