http://www.sigma-gen...alc/DNACalc.asp
Why SIGMA calculator (above) different from the formula below?
Tm= 2(A+T)+4(G+C)
Any tips will be appreciated.Thanks!
Primer Tm calculation.
Started by Ic3_88, Mar 23 2012 07:11 AM
1 reply to this topic
#1
Posted 23 March 2012 - 07:11 AM
#2
Posted 23 March 2012 - 08:40 AM
the formula you give is very general, rough "rule of thumb" and will give you a "ballpark" figure.
the calculator uses more precise values for the various bases and may be taking other factors into consideration (i can't list those factors because i don't use the sigma calculator, this statement is for the calculators in general).
the calculator uses more precise values for the various bases and may be taking other factors into consideration (i can't list those factors because i don't use the sigma calculator, this statement is for the calculators in general).
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genius does what it must
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