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Primer Tm calculation.


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#1 Ic3_88

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Posted 23 March 2012 - 07:11 AM

http://www.sigma-gen...alc/DNACalc.asp


Why SIGMA calculator (above) different from the formula below?


Tm= 2(A+T)+4(G+C)


Any tips will be appreciated.Thanks!

#2 mdfenko

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Posted 23 March 2012 - 08:40 AM

the formula you give is very general, rough "rule of thumb" and will give you a "ballpark" figure.

the calculator uses more precise values for the various bases and may be taking other factors into consideration (i can't list those factors because i don't use the sigma calculator, this statement is for the calculators in general).
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