Posted 22 March 2012 - 11:16 PM
I am trying to amplify a 3 kb cDNA. the genome of my interest is AT rich (77%).I tried amplifying it using Pfu polymerase,incresed Mg conc upto 3mM, decreased the extension temperature to 68C,I reduced the annealing temperature to as low as 45oC but i fail to get an amplification.
Kindly suggest how do i get over it..
awaiting for a solution
Posted 23 March 2012 - 12:59 AM
Posted 23 March 2012 - 04:21 AM