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question about differentation of THP-1

THP-1 PMA differentation Macrophage

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#1 BMF



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Posted 22 March 2012 - 04:38 PM

In the lab I am working, THP-1 Cells differentiated by PMA to Macrophage cell just for 24 hour. That is:
we add PMA to THP-1 Cells and after 24 hour we change the media and without any delay we also treat the cells by our compound of interest to see changes in the cell signalling.

I have read in some papers that I should let the cells to be differentiated for 72hr before doing any next step.

Also tagged with one or more of these keywords: THP-1, PMA, differentation, Macrophage

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