Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

question about differentation of THP-1

THP-1 PMA differentation Macrophage

  • Please log in to reply
No replies to this topic

#1 memari

memari

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 148 posts
11
Good

Posted 22 March 2012 - 04:38 PM

In the lab I am working, THP-1 Cells differentiated by PMA to Macrophage cell just for 24 hour. That is:
we add PMA to THP-1 Cells and after 24 hour we change the media and without any delay we also treat the cells by our compound of interest to see changes in the cell signalling.

I have read in some papers that I should let the cells to be differentiated for 72hr before doing any next step.
-----
Babak Memari





Also tagged with one or more of these keywords: THP-1, PMA, differentation, Macrophage

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.