Fugene 6 + serum free DMEM + problem
Posted 22 March 2012 - 04:25 PM
What if I had mixed the remaining necessary Fugene 6 (.5 ul/ well, I calculated) with Serum Free DMEM, let it sit for 5 minutes, and then dispensed it straight into the wells that already contain a Fugene 6 + DNA + SF DMEM? I'm guessing it wouldn't work, since it's not on any protocol, but what would it have done? Or would my results have been more messed up than they will be?
Does this question make sense?
Posted 25 March 2012 - 11:54 AM
Posted 31 March 2012 - 04:47 AM