Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Plasmid vector for yeast and E. coli

vector selection yeast human protein E. coli cloning

  • Please log in to reply
2 replies to this topic

#1 anaivan

anaivan

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 22 March 2012 - 12:21 PM

Hi,
I am looking for a good plasmid vector system to express a human protein of (with N terminal His tag) in both yeast and E. coli. The protein doesnt have much post translational modifications except couple of acetylation in internal lysine residues. I will be synthesizing the gene; hence it doesn't really matter if the vector has His tag in it or not. The gene insert will be 1000 bases approx.

Is there any vector which can be used in both systems? (I doubt it though.) Please suggest me a vector for both systems which worked great for you. I would prefer if they have commonly used antibiotics such as kanamycin, etc. Please let me know the typical yield you get with such system, if you dont mind. I would appreciate your help/suggestion.

Also, do you think acetylation might be signification for protein's activity? The protein's function is hydrolysis.

Thanks!

Edited by anaivan, 22 March 2012 - 01:08 PM.


#2 pDNA

pDNA

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 493 posts
14
Good

Posted 26 March 2012 - 12:49 PM

Clonetech should have some ...e.g. pGBK T7

No comments on yield ...i do not work with yeasts!

Regards,
p

#3 anaivan

anaivan

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 27 March 2012 - 09:15 AM

Thanks pDNA, for your reply.





Also tagged with one or more of these keywords: vector selection, yeast, human protein, E. coli, cloning

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.