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normalization to protein content or cell number


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#1 zienpiggie

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Posted 22 March 2012 - 09:26 AM

Hi everyone,

I need to determine the amount of my compound in my treated cells, and I realize that some express their compounds being normalized to protein level or per cell number (e.g. per a million cell). I am wondering if there is a reason to favour one over the other? I am leaning towards expressing it per protein just because expressing per cell number by cell counting might probably give a greater variation as opposed to using bradford assay. But then I'd like to know if anyone else have insights regarding the topic.

#2 bob1

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Posted 22 March 2012 - 11:52 AM

Either should be fine. If your counting is accurate enough (or you have a machine to do the counting for you), I find that this is easier to compare to in terms of real usable numbers i.e. ones I can imagine/visualise.

#3 zienpiggie

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Posted 23 March 2012 - 02:38 PM

Thank you Bob1!




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