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Message Amount in qPCR

qPCR Real Time PCR mRNA

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#1 rgbaby



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Posted 21 March 2012 - 02:44 PM

As you know when doing qPCR, you do a ratio of some housekeeping gene Ct to the Ct of your target message and compare levels between changes in that message over time, induction, etc. I want to know if anyone has data on the amount of message of GAPDH preferably in HEK293 cells? Is it 50 copies per cell? 1000 copies per cell? Just a mere ratio will not do it for me.


#2 Trof


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Posted 22 March 2012 - 03:48 AM

I'm affraid that can differ between batches of cell line. You can do absolute quantification to find out on your sample.

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