Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Message Amount in qPCR

qPCR Real Time PCR mRNA

  • Please log in to reply
1 reply to this topic

#1 rgbaby

rgbaby

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 21 March 2012 - 02:44 PM

As you know when doing qPCR, you do a ratio of some housekeeping gene Ct to the Ct of your target message and compare levels between changes in that message over time, induction, etc. I want to know if anyone has data on the amount of message of GAPDH preferably in HEK293 cells? Is it 50 copies per cell? 1000 copies per cell? Just a mere ratio will not do it for me.

Thanks.

#2 Trof

Trof

    Brain on a stick

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,200 posts
109
Excellent

Posted 22 March 2012 - 03:48 AM

I'm affraid that can differ between batches of cell line. You can do absolute quantification to find out on your sample.

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

I never trust anything that can't be doubted.

'Normal' is a dryer setting. - Elizabeth Moon






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.