Hi Guys,
So i wanted to see if you guys could help me in recommending good loading control antibodies. for the last few weeks i have been trying to troubleshoot my blot and wondering what could be the reason i was obtaining so many bands and i have been using Monoclonal Anti-Actin, Clone AC-40 from sigma, and i have tried several dilutions and i still obtain many bands so at this point looking to try a different primary.
1 reply to this topic
Also tagged with one or more of these keywords: western, antibodies
![]() |
Protocols and Techniques Forums →
SDS-PAGE and Western Blotting →
3 extra bands show up on my western after an IPStarted by Guest_DrZeus_* , 07 May 2013 |
|
|
|
Protocols and Techniques Forums →
General Lab Techniques →
Electrophoresis →
Western blot- bands weakStarted by Guest_sdheeru_* , 28 Feb 2013 |
|
|
||
Protocols and Techniques Forums →
Immunology →
Serum inactivation:Started by Guest_@bhijit_* , 20 Nov 2012 |
|
|
||
Protocols and Techniques Forums →
SDS-PAGE and Western Blotting →
HELP!! Anti-GFP antibody resuspended in 20% SDS!!!!Started by Guest_SPombe3_* , 24 Oct 2012 |
|
|
||
Protocols and Techniques Forums →
Molecular Cloning →
about a bookStarted by Guest_majidatf_* , 12 Oct 2012 |
|
|














