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cDNA concentration of qPCR

cdna concentration qpcr

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#1 thinker



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Posted 20 March 2012 - 01:38 PM

Which the minor concentration of cDNA that can I use in qPCR?
And which would the best concentration?

#2 Curtis


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Posted 20 March 2012 - 05:53 PM

you need to look at the manual of your DNA polymerase. It must recommend a certain amount. The lowest could be 1 pg, but normally people use higher concentrations. we normally keep it below 1 ug because usually above that gives us smear on the gel.

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